![]() Have a suggestion on newsletters you’d like to see in next year’s update? Make a submission via our contact page. ![]() As always, all our recommendations are genuine, unbiased, and based on our actual experiences. ![]() Subscribers call it the best resource on Value Investing in India. Check out the latest issue here and subscribe here. VIA is a monthly, premium newsletter that brings to you the best and biggest ideas in. A wealth of coverage on what the big, established players are releasing next instead of reporting. In their own words There’s a focus on the amount of money raised instead of innovation and quality of a product or service. Newsletters are organized into the following categories:īy the way, some of the links in this article may be from partners or affiliates - which simply means we may get to make money at no cost to you. The best part about this newsletter is the brief and succinct summary given below each story link. The SaaS Weekly is a weekly newsletter covering the major SaaS news from around the globe. Our team collected and curated the best niche newsletters that are actually worth subscribing to.Įxplore this list, subscribe to any of the newsletters that stand out to you, and fill your inbox with a bit of goodness. 11 Best Literary & Books Newsletters to Subscribe (FREE) The Book Satchel The best bookish and literary newsletters for book recommendations, and essays. Our email addresses are sacred - and only the best newsletters deserve to get access to our inboxes. A great newsletter can brighten your day, help you learn, make the world easier to understand, or empower you to make a difference.Īt the same time, we’re all receiving way too many emails as it is. The Skift India Newsletter is your go-to platform for all news related to travel, tourism, airlines, and hospitality in India. Our inboxes deserve to house more than just work emails.
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7/31/2023 0 Comments Rad dad zine![]() ![]() Parenting-themed zine with writing by Tomas Moniz, Dave Gilbert, David Cahn. Genres include Riot Grrrl, Radical Politics, Parenting, Gender & Sexuality, Comics/Art/Photography, DIY Skill-Sharing, and more. RAD DAD 19 parenting zine Microcosm ARTNOOSE Tyler Cohen small press 2011. Rad Dad, a Zine for Rad Dads, Hip Mamas, and Everybody In-between. Other featured installations from local zinesters include Endless Canvas, MariNaomi, Jaime Crespo, and others, and tons of other zines on a display designed by Jamie Mayne of RPS. crew – it will be available for purchase at R.P.S., Actual Cafe, and other art spaces and book outlets when it’s done. A compilation of thoughtful essays by parents who break the mold, Rad Dad contributors question mainstream parenting norms, gender stereotypes, and exactly what it means to take on the task of bringing up the next generation of people. The zine will be printed and distributed by the R.P.S. DJ Baro added some vinyl flavor to the party.ĭuring the opening party visitors drew pages to be compiled into th Paper Trails Zine. The opening featured a Zine Variety Show with readings by Tomas Moniz, John Bobst, and Mari Naomi along with music performances by Annah Anti-Palindrome and Jon Barba. It’s a zine-themed show, encompassing hundreds of zines, many of which are locally-produced. Paper Trails is a collaborative art show – partnering with the Rock Paper Scissors Collective and Tomas Moniz of Rad Dad Zine. Show runs from March 9th through April 12th, 2012. ![]() "E' ufficiale: l'Europa League e la Conference League sbarcano su DAZN". "Virgin Media to broadcast Champions League match on Wednesday nights". ^ "Virgin Media Ireland secures rights to UEFA Champions League, UEFA Europa League, UEFA Europa Conference League for the next three years"."Emtek Group Perpanjang Hak Siar UCL dan UEL Selama 3 Musim Ke Depan". ^ Arie Harnoko, Rizqi (18 January 2021). ![]() "Exclusive: Sony renews UEFA broadcast rights in India for next 3 years". ^ "Meistaradeildin afram á Stöð 2 Sport í samstarfi við Viaplay".^ "NENT Group to show UEFA Champions League on Viaplay in Iceland".^ "Visszatér a futball az RTL Magyarország képernyőjére!".^ "Történelmi megállapodást kötött az MTVA és az Európai Labdarúgó Szövetség".^ "PCCW Media and beIN Sports bring UEFA Champions League toNowTV customers from 2021/22 to 2023/24 seasons" (PDF).^ "UEFA Champions League and UEFA Europa League on COSMOTE TV until 2024".^ "Rechte-Coup: Europa League bis 2024 bei RTL, NITRO und TVNOW"."Europa League returns to M6 as French sales process complete". ^ "RMC Sport DIffuseur des Quatre Coupes D'Europe de Football Pour Les Trois Prochaines Saisons".^ "Le Groupe M6 Acquiert Auprès de L'UEFA Les Droits de Diffusion de L'UEFA Ligue Europa et de L'UEFA Ligue Europa Conference"."Canal Plus completes hat-trick with Europa League package". ^ a b c "NENT Group signs new media rights agreement with UEFA".^ "TV 2 sikrer sig rettighederne til Europa League".Přibude navíc Evropská konferenční liga". ^ "Evropská liga zůstane na České televizi.^ a b "Stanice Sport1 a Sport2 budou od září vysílat Evropskou ligu UEFA a zcela novou Evropskou konferenční ligu UEFA".^ "Saran Media Group retains Uefa club competition rights in Central Asia".^ a b c d e f g h i j k l m n o p q r "Where to watch the UEFA Europa Conference League: TV broadcast partners, live streams".^ "The most prestigious European club football tournaments - in the channels of bTV Media Group until 2024"."Thailand added in beIN's Southeast Asia deal for Uefa club competition rights". ^ a b c d e f Ross, Martin (23 June 2021).^ "TV Cultura vai transmitir a temporada 2021/22 da Liga Europa da Uefa".^ "SBT repassa à TV Cultura transmissão da Europa League".^ "Disney vence leilão e fecha acordo até 2024 pela Liga Europa na TV paga". ![]()
7/31/2023 0 Comments Fireside dining deer valley resortThe outing covers 1.5 miles and takes roughly one hour, wrapping up at the front doors of the restaurant.Įasily managed wide ski slopes, snowshoeing to Dinner at Deer Valley is for all The snowshoe tour will embark from the Silver lake Village where you will quickly leave the lights behind as you traverse the serene slopes. After a scenic snowshoe, the raclette or roasted leg of lamb stations are a must. With 4 large fireplace stations featuring different courses, there are options for everyone. This ski lodge is transformed into fireside dining, a European dining experience not to be missed. W asatch Brew Pub (at the top of Main Street great for watching sports games too)īutchers (a nicer restaurant that’s also kid-friendly we recently ate in one of the heated snow globe/yurt things outside the restaurrant and it was awesome)ĭeer Valley Resort (any of the restaurants on the mountain are great.Experience Empire lodge after the hustle and bustle of the daily lunch rush. Squatters (a little outside Main Street great food but always packed so go early) Main Street Pizza and Noodle (on Main Street) Grappa (super romantic – I recommend getting a sitter if you have the kiddos with you but all the Park City restaurants will work with kids)ĭavanza’s (Andrew’s favorite pizza spot – get the bbq chicken right in town) Regis Bar (adults only) or Mountain Terrace (great ski in/ski out option and apres too)īrass Tag Best “Nicer” Restaurants for Dinner ![]() Harvest (on Main Street or at Park City Mountain)Īpex at Montage (for a fancier breakfast or lunch)ĭaly’s Pub and Rec (the best for kids it’s right on the mountain and they have bowling and arcade games, making it one of my favorite things to do in Park City with kids)ĭeer Valley Resort (any of the restaurants are great and perfect for breakfast or lunch pre/post skiing the turkey chili and blueberry mojitos are a must-have) The Caledonian (if you want to stay right on Main Street in town there’s a town lift that takes you to Park City so it works if you want to ski too) Best Breakfast and Lunch Spots Rent a House on Deer Valley Mountain (I prefer skiing Deer Valley and they have tons of great rentals you can book that are great for families only downside is that the hotel/condo rentals offer more full service, from rides to ski valet that you don’t necessarily get with a house) The Lodge at the Mountain Village (if you’re looking for ski in/ski out at Park City Mountain that’s family-friendly and more affordable especially at peak times can’t beat the convenience) Regis (I would stay here if you’re traveling without the kiddos) Lodges at Deer Valley (great for families – perfect compromise of a home and hotel) I seriously couldn’t recommend Park City enough, whether you’re getting away as a couple or as a family, heading there during the ski season or the summertime or fall. I included tons of things to do in Park City with kids, since that was my most frequent question in addition to requests for where to eat with kids, where to ski, and where to stay. Park City is truly a magical spot, and I’m so excited to finally put together my Park City guide to share with those of you who are considering or planning to check it out. ![]() Really the only time of year I’d recommend avoiding the area is late spring when the ski mountains close and it’s slushy and a bit dreary. We also love the area in the summertime, when it’s beautiful and there are so many fun outdoor activities. Christmas is one of my favorite times to go to Park City because it’s a magical wonderland and such a special spot to celebrate the holidays, but before kids, we also loved to go out in late March for spring skiing when the powder never stops and it’s warm and sunny in the afternoons. ![]() We also got engaged on Deer Valley mountain and married at Sundance Resort, which is just outside of Park City, so we have lots of good Park City (and beyond) recommendations, from restaurants to shopping to activities. But Andrew and I came to know the area inside and out after living out there for a year in our early 20s. My husband’s family has been going out there for over 30 years, so we’re very familiar with the area. We go to Park City every year for Christmas with my husband’s family. well, ask and you shall receive and it seems a lot of you are going to my favorite winter (and honestly, summer and fall) destination with your kiddos. I included all the things to do in Park City with kids because…. Ever since our annual Christmas vacation in Park City, Utah, I’ve gotten so many requests for Park City recommendations and info on the area, so I thought I’d put together a blog post. 7/31/2023 0 Comments Intel graphics command centerThe Application Controlled setting sets VSync under control of the game or application that uses it. NoteĪlways On sets VSync to always enabled. Find the Vertical Sync setting and apply the desired setting for the game or application.Click Custom to create a custom profile.If the game does not appear under My Games, add the game by following the steps provided in How to Add Games in the Intel® Graphics Command Center. In the Home tab, s elect the game or application listed under My Games to edit the settings.Click the Intel Graphics Command Center icon to open the application. From the Windows Start Menu, search for Intel Graphics Command Center.Open the Intel® Graphics Command Center:.In order to configure VSync in Intel GCC, follow these steps: ![]() VSync can be set to Always On, Always Off, or Application Controlled. Select the Intel® user interface used by your system for steps to enable or disable this feature.Ĭlick or the topic to expand the content: Intel® Graphics Command Center (Intel ® GCC) ![]() In older Intel® graphics drivers, this feature could be disabled for OpenGL* applications (not DirectX* games/applications). When VSync is off, you get virtually unlimited Frames Per Second (FPS) but depending on your configuration, screen tearing, jerkiness or stuttering is more likely. When VSync is on, you get perfectly aligned frames with no glitching. If it's not synchronized, it can cause screen tearing, an effect that causes the image to look glitched or duplicated horizontally across the screen. Vertical Synchronization (VSync), helps create stability by synchronizing the image frame rate of your game or application with your display monitor refresh rate. Each of those special students should have an opportunity to say something if you are doing the graduation as an individual class. We all know that parents are going to be looking at one person: their child. I have spotted an occasional third grader sporting this kindergarten keepsake. The shirts are always over-sized, and they wear them proudly for many years to come. Each individual drawing is placed on the shirt to make a classroom portrait. Students draw their faces on a small square of paper. I saw this idea in a newspaper article, and I had to use it. T is for treats that we sometimes earned.Į is for excitement. R is for remembering everything we learned. We make more every day.Ī is for the alphabet we know how to say. We know, one two three.Į is for exercise to keep our bodies strong. Luckily, the word Kindergarten is long This allows two students to hold up one letter in the word. If you have a Cricut, you can make letters in the word to be held up. Paint Reach For the Stars in big letters and paint or punch out some stars to go along with the hand prints. Īll you need is butcher paper and some paint and a bunch of little hands. Having the students place their hand prints on the banner helps each child to play a bigger part in the celebration. Making a classroom banner to display at kindergarten graduation is a fun decoration that your students will love. Parents will be extremely touched by this number. It is very beautiful when the kids sing it with the sign language actions. They cannot contain their enthusiasm when they say, “Left, right, left right.” The students also love this kindergarten graduation song/chant. I love this one as it is so easy for the kids to perform because they are just imitating what you say. Other great kindergarten graduation songs are KinderFunk, Time to Graduate, We are the Champions. This song is a timeless favorite.Īlso, kindergarten students doing a kick line is priceless. It is so adorable that I asked her for a copy of the words. I found this song when my daughter’s kindergarten teacher used it for her kindergarten graduation. First Grade, First Grade (New York, New York) The music to this song fits the lyrics perfectly. Barton,” but “ Kindergarten, Kindergarten” is just as cute. Kindergarten, Kindergarten (Love and Marriage)īecause my last name is Barton, we sing, “Kindergarten with Ms. ![]() Regardless, everyone is impressed with this kindergarten graduation song. Or, maybe it is because it lends itself to the cutest movements ever. Maybe, it is because I love John Travolta. Here are my favorite kindergarten graduation songs and ideas. ![]() Parents always compliment me on the fun songs that we sing for graduation. I am not sure who it was that made up the words to these songs, but I want to thank them. The parents know the songs, and who are we kidding? This day is really for them. I like to use the popular kindergarten graduation song parodies made exclusively for this event. The kindergarten graduation songs can add to the celebratory and memorable atmosphere. Kindergarten graduation is the first real milestone that children have in school. 7/31/2023 0 Comments Wine rack barIf you do nothing, you will be auto-enrolled in our premium digital monthly subscription plan and retain complete access for $69 per month.įor cost savings, you can change your plan at any time online in the “Settings & Account” section. For a full comparison of Standard and Premium Digital, click here.Ĭhange the plan you will roll onto at any time during your trial by visiting the “Settings & Account” section. Premium Digital includes access to our premier business column, Lex, as well as 15 curated newsletters covering key business themes with original, in-depth reporting. ![]() Standard Digital includes access to a wealth of global news, analysis and expert opinion. Complete your bar room with bar seating, fun gaming stations like a pool table and cozy lighting from lamps or wall sconces.During your trial you will have complete digital access to FT.com with everything in both of our Standard Digital and Premium Digital packages. With places to store wine bottles, glassware and miscellaneous bar accessories, this cabinet becomes the perfect centralized spot to mix drinks for at-home parties and events. Mix-and-match the different bar elements to create your perfect home bar station, then complete the look with a set of bar stools and a small table.īrowse the collection of wine rack cabinet furniture at Pottery Barn to furnish a gorgeous bar area in your home. You can then pair the base with open hutches or cabinet hutches. Many can be configured to allow the doors to open to the right or left. The modular bar furniture collection includes a variety of different cabinet bases with glass doors, opaque doors or wine grids. Sometimes the only way to get exactly what you want is to build it yourself.
7/30/2023 0 Comments New vegas old world blues![]() One down side of the content is it is rare to come by a stim-pack and parts can become very difficult, so you will have to rely on food as well as drinks to keep your health up. But it all comes down to the end when you realize your own brain is much smarter than you think it is. ![]() The juke-box can equip one of your new weapons with specific emitters and the auto-doc can even give you permanent implants that give you bonuses. All of these appliances though, even the very angry talking toaster, have special things they can do but all require you to go on a scavenger hunt to find their missing parts in order to do them. They all talk as well and the light are in an eternal quarrel, so I would stay clear of them. Of course there is the suit that talks to you and room full of appliances that all have their own personalities. Other than that you pretty much do what you usually do in Fallout, with its typical missions. race I suppose you could say but the real fun in this third DLC installment is the interactions you have with these things, they are hilarious and they don’t even know it. The inhabitants are trapped in fear of a Dr. You will come across force fields, robot scorpions and of course the main inhabitants who were able to sustain them selves during the fallout, just certainly not in the way you would expect. ![]() The whole area here is unique and scientific looking as some of the weapons show some true technological advancements whether it comes to a plasma gun or a lead chucking machine. You actually get perks for running around with out them! I’ll stop there on that subject for people that really want to be surprised. You’ll travel across this previously unknown place, completing mission and you don’t even need things like your brain, spine or heart. Once again the level cap has been risen by five levels which really makes it more worth it at you try to max out your skills. ![]() We found it for real and it’s not so pretty. Just to add this one thing that people may remember going to a 1950’s black and white world as a child in the first game being forced to do bad things in a neighborhood. that is the best I have got with out giving away any spoilers. You will come across new enemies and new, well I’m not really sure what I would call them… interestingly, odd beings? I don’t know. New groovy apparel, new weapons as well as useless coffee mugs (well useless to some) are all apart of the latest addition in the Fallout: New Vegas series. This DLC also brings you back to the real roots of exploration, which is a large part of what Fallout is. And while the Fallout series has always had its smart remarks, I truly believe this content to be one of the humorous pieces of content I have ever played in the world of Fallout. Not an alien world kind of place, a more humorously, previously unknown place. I won’t give any spoilers away but let’s just say after that I was transported to another place. Then all of a sudden a huge beam of light flashed before me, almost blinding me and the story of ‘Old World Blues’ began. After the unsatisfactory of the thrilling eyeball on screen, thinking I would see a film or something I went to tinker inside the projector. By this time the projector lit up the screen wilth a strange and very non-entertaining eyeball. I sat there waiting and waiting, drank a redbull, waited some more, drank a beer, waited, started to get frustrated and then finally it was midnight in Fallout: New Vegas time. In the broadcast it said to arrive early and i guess I arrived a little too early. A few enemies were waiting for me there but I had no problem taking them out. The projector was an old broken down United States of America Satellite clearly marked. As I loaded up Fallout: New Vegas, I received a strange radio signal on my pip-boy, essentially inviting me to a midnight showing of a feature film of some sorts that had the reminiscence of a drive in movie theater. 7/30/2023 0 Comments Jimmy butler stats vs celtics 2022![]() Butler grabbed the offensive rebound and converted a layup, putting Miami ahead for just the second time in the game. Then, the game turned on its head after halftime.Ī minute into the third quarter with the Heat trailing the Celtics by a point, Gabe Vincent’s three-point attempt bounced off the rim. In the first half, Boston led by as much as 13 and went into halftime 62-54 up with forward Jayson Tatum tying a career playoff-high in scoring for one half as he contributed 21 points. He put us on his back,” Heat guard Tyler Herro said after the game.ĭespite losing two of their starters – Marcus Smart and Al Horford – hours before the tip-off, the Celtics kicked off Game 1 strongly with a 7-0 run. All other options, cap holds, exceptions are assumed as declined, waived or renounced.Jimmy Butler poured in 41 points, nine rebounds, five assists, four steals and three blocked shots – numbers never seen before in an NBA playoff game – as he led the Miami Heat to a 118-107 comeback victory over the Boston Celtics in Game 1 of the Eastern Conference Finals. This is calculated by determining all guaranteed salaries + any dead cap from non-guaranteed salaries. The Miami Heat coach was his usual controlled self after guiding his team to a 118-107 victory over the Boston Celtics, fueled by Butler's greatness, to take a 1-0 lead in the NBA Eastern. Cap TypeĢ023-24 Practical Cap Space Available Practical Cap Space: The team may only sign outside players using any available exceptions or at league minimum salaries. This means Cap Holds & Exceptions are NOT included in their Total Cap Allocations, and renouncing these figures will not afford them any cap space. The Heat are currently over the league salary cap. Teams that spend over the threshold pay fines (estimated below). Stats Games 1 Miami 53 - 29 Eastern Conference Finals BOS wins 4-3 View Bracket 2 Boston 51 - 31 Game 1: MIA leads 1-0 107 Final 118 Game Recap Game 2: Series tied 1-1 127 Final 102 Game Recap. Typeĭenotes the Heat current standing in terms of the luxury tax threshold. The "credit" balance remains as an exception that can be used within the next year to acquire salary via trade without further relinquishing additional salary. Trade Exception Details becomes available when a team relinquishes more salary than it acquires during a trade. Taxpayer Mid-Level Exception Details is awarded annually to teams who are above the cap and above the Luxury Tax Apron ($172,346,000) and can be used for contracts up to 3 years in length. Heat injury report: Probable: Herro (ankle), Highsmith (ankle), Robinson (ankle), Strus (shoulder). Please disable your ad blocker to view the video content. Butler had 41 points, nine rebounds, five assists. Exceptions can be split up for multiple player signings, however only one of these exceptions may be used in a season and they cannot be combined. Ira Winderman IraHeatBeat Jimmy Butler upgraded to questionable for Friday at Celtics. WS: Jimmy Butler (3.8) On this page: An ad blocker has likely prevented this video content from loading. Butler once again did plenty of everything, leading the Heat to a 118-107 victory against the Celtics in Game 1 of the Eastern Conference finals. Player (5)Įxceptions are essentially "grants" offering teams an additional way to sign players. These figures are not counted toward a team's Luxury Tax Allocation. Renouncing these cap holds will NOT afford the team any new cap space. Since the Heat are currently over the league salary cap, these cap holds do not count against their Total Cap figure. ![]() Finals MVP: Stephen Curry (31.2 / 6.0 / 5. Heat League Champion: Golden State Warriors. Player (2)Ĭap holds represent "placeholders" for pending free agents, unsigned draft picks, offer sheets, etc. 2022 NBA Eastern Conference Finals Celtics vs. Two-way salaries do not count against a team's Total Cap/Tax Allocations. Players signed to Two-Way contracts that are currently designated to G-League affiliate. 7/30/2023 0 Comments Antibody production cellsGangwar N, Mishra R, Budholiya N, Rathore AS (2021) Effect of vitamins and metalions on productivity and charge heterogeneity of IgG1 expressed in CHO cells. įreilich R, Arhar T, Abrams JL, Gestwicki JE (2018) Protein-protein interactions in the molecular chaperone network. Įszterhas SK, Bouhassira EE, Martin DI, Fiering S (2002) Transcriptional interference by independently regulated genes occurs in any relative arrangement of the genes and is influenced by chromosomal integration position. ĭoronina VA, Wu C, de Felipe P, Sachs MS, Ryan MD, Brown JD (2008) Site-specific release of nascent chains from ribosomes at a sense codon. ĭe Nardis C, Hendriks LJA, Poirier E, Arvinte T, Gros P, Bakker ABH, de Kruif J (2017) A new approach for generating bispecific antibodies based on a common light chain format and the stable architecture of human immunoglobulin G(1). ĭahodwala H, Lee KH (2019) The fickle CHO: a review of the causes, implications, and potential alleviation of the CHO cell line instability problem. Ĭromwell ME, Hilario E, Jacobson F (2006) Protein Aggregation and Bioprocessing. Ĭhennamsetty N, Helk B, Voynov V, Kayser V, Trout BL (2009) Aggregation-prone motifs in human immunoglobulin G. īuchanan A, Clementel V, Woods R, Harn N, Bowen MA, Mo W, Popovic B, Bishop SM, Dall’Acqua W, Minter R, Jermutus L, Bedian V (2013) Engineering a therapeutic IgG molecule to address cysteinylation, aggregation and enhance thermal stability and expression. īrinkmann U, Kontermann RE (2017) The Making of Bispecific Antibodies. īorth N, Mattanovich D, Kunert R, Katinger H (2005) Effect of increased expression of protein disulfide isomerase and heavy chain binding protein on antibody secretion in a recombinant CHO cell line. īickel F, Herold EM, Signes A, Romeijn S, Jiskoot W, Kiefer H (2016) Reversible NaCl-induced aggregation of a monoclonal antibody at low pH: characterization of aggregates and factors affecting aggregation. īayat H, Hossienzadeh S, Pourmaleki E, Ahani R, Rahimpour A (2018) Evaluation of different vector design strategies for the expression of recombinant monoclonal antibody in CHO cells. īarzadd MM, Lundqvist M, Harris C, Malm M, Volk AL, Thalén N, Chotteau V, Grassi L, Smith A, Abadi ML, Lambiase G, Gibson S, Hatton D, Rockberg J (2022) Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells. Reducing the aggregations can improve the quality of recombinant antibodies.Īlam ME, Slaney TR, Wu L, Das TK, Kar S, Barnett GV, Leone A, Tessier PM (2020) Unique impacts of methionine oxidation, tryptophan oxidation, and asparagine deamidation on antibody stability and aggregation.Intracellular environment and extracellular parameters influence recombinant antibody aggregation.The recombinant antibody aggregation in mammalian cell systems is reviewed. ![]() In this review, we discuss recent progress in the field of RTAs aggregation, with a focus on factors that cause aggregation during RTA production and the development of strategies for overcoming RTA aggregation. Therefore, the mechanisms underlying RTA aggregation and measures for avoiding aggregation are interesting topics in RTAs research. RTA aggregations are especially concerning as they can trigger human immune responses in humans and may be fatal. A major limitation associated with the use of RTAs is their aggregation, which can be caused by a variety of factors this results in a reduction of quality. Recombinant therapeutic antibodies (RTAs) are among the most important and promising RTPs for biomedical applications. Nowadays, most RTPs approved for marketing are produced in Chinese hamster ovary (CHO) cells. ![]() In the HAT medium, aminopterin, a dihydrofolate reductase inhibitor, is used to inhibit de novo nucleotide synthesis pathway, while exogenous hypoxanthine and thymidine are supplied to ensure DNA synthesis can be carried out via the salvage pathway.Mammalian cell lines are frequently used as the preferred host cells for producing recombinant therapeutic proteins (RTPs) having post-translational modified modifications similar to those observed in proteins produced by human cells.
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